Enumeration of intestinal
enterococci in 24 hours
ENTEROKAT® is a fast and easy-to-use test system for the detection and enumeration of intestinal enterococci (genus Enterococcus) in water samples. The test is suitable for a wide range of waters, especially drinking water, bathing water and similar with high background bacterial counts that might include an appreciable amount of suspended matter. It allows the detection of enterococci at 1 CFU / 100 ml with definitive results within in 24 ± 2 hours in the presence of heterotrophic bacteria in numbers as high as 1 × 10^6 per 100 ml sample.
- Optimized workflow in your laboratory as compared to membrane filtration: Less than one minute of sample processing time.
- Quantification with the same enumeration trays and sealing devices used for COLIKAT RAPID®
- Sample evaluation after ± 24 hours.
- Detection and enumeration of enterococci for a wide range of the water matrix, including waters with high background flora and suspended matter.
- High sensitivity with lower limit of detection at 1CFU per 100 ml.
- Enumeration of intestinal enterococci (genus Enterococcus) in water samples, acc. to upcoming EN ISO 7899-3.
Product benefits:
- The ENTEROKAT® reagent dissolves significantly faster in the sample.
- Improved shelf life of 24 months at 2–30 °C (and minimum shelf life after initial opening of stand-up pouch of 6 months).
- No antifoam is needed for the majority of water samples.
- No UV cabinet needed due to clear colour change from blue to green.
- Saves more than 80% volume of waste and storage area for the ENTEROKAT® reagent.
- Manufactured and distributed by your reliable service partner Xebios Diagnostics Group GmbH and our local established and experienced distribution partners.
Tap Water
Bottled Water
Bathing Water
Waste Water
Surface Water
Test Principle
ENTEROKAT® utilizes Ortho-Nitrophenyl-ß-D-glucoside as a nutrient indicator and incorporates a specifically designed blue background color in its formulation. When the substrate is metabolized by intestinal enterococci, the sample turns from blue to green to indicate positive detection. After incubation, sample wells (or tubes) that have a green color are considered positive for intestinal enterococci.
As compared to the conventional detection and enumeration of intestinal enterococci by the membrane filtration method according to EN ISO 7899-1 the ENTEROKAT® test system offers multiple advantages:
- Optimized workflow in your laboratory as compared to membrane filtration: Less than one minute of sample processing time.
- Quantification with the same Enumeration Trays and sealing devices used for COLIKAT RAPID.
- Sample evaluation after 24 hours.
- Detection and enumeration of enterococci for a wide range of water matrices, including waters with high background flora and suspended matter.
- High sensitivity with lower limit of detection at 1 CFU per 100 ml.
Application – Instructions for use
Presence / Absence: Add 1 bag of ENTEROKAT® to 100 ml of water sample. Close the sample vessel and shake until the reagent is completely dissolved.
For presence / absence testing incubate the closed vessel for 18-22 hours at 36°C +- 2°C and read-off the result. The vessels should have room temperature before incubation. If turned yellow coliforms are present. If it shows fluorescence under UV-light Escherichia coli is present.
Add 1 bag of ENTEROKAT® to 100 ml of water sample. Close the sample vessel and shake until the reagent dissolves.
For quantitative testing pour the 100 ml sample into the Enumeration Tray.
Seal the Enumeration Tray with the COLIKAT® Enumeration Tray Sealer or another enumeration tray sealing device.
Incubate the sample 18–22 hours at 36 °C ± 2 °C. Count the yellow wells of Enumeration Tray (= coliforms). Count the yellow wells of the Enumeration Tray that show fluorescence under an UV light (365m) in a dark environment (= Escherichia coli).
Transform the count results to the number of CFU in the sample by using the MPN table in the appendix to EN ISO 9308-2.
Quality
The manufacturing, storage and quality control of ENTEROKAT® is carried out by Xebios Diagnostics Group GmbH in compliance with EN ISO 11133 and considering Good Manufacturing Practice (GMP). The quality management system is ISO 9001 certified. The quality testing of ENTEROKAT® takes place in our EN ISO 17025 accredited laboratory complying with all requirements of EN ISO 11133 and EN ISO 9308-2. It is our goal to meet our customers’ high quality requirements at all time.
Our quality management system includes broad testing procedures for sterility, stability and shelf live, the microbiological performance and physical condition of our products. We trust only established partners which have proven to delivery according to our high quality standards for years for the sourcing of our raw materials and technical equipment.
Distribution Partners
Germany
Switzerland
Italy
United Kingdom
Czech Republic
New Zealand
Norway, Sweden, Finland & Estonia
Australia
South Africa
Your country is not yet listed and you are interested in Distributorship: Please contact us at sales@xebios.com.
Contact and Support
Please contact us at any time for all your questions about our products and services as well as for an individual quotation.
You can reach our team by phone, e-mail or by using our contact form below.
Xebios Diagnostics Group GmbH
Telleringstraße 49
40597 Düsseldorf
T : +49 211 78 88 07 – 00
E : sales@xebios.com
COLIKAT® RAPID is a fast test to simultaneously detect both total coliforms and Escherichia coli in water samples providing the test’s results within 18 hours. COLIKAT® RAPID is suited for the entire water matrix.
